Genomic Precision

 A SeraCare blog focused on precision medicine and advanced clinical diagnostics

Extracting Formalin-fixed, paraffin-embedded (FFPE) nucleic acids for NGS

Answering frequently asked questions (FAQs)

Posted by Yves Konigshofer on Mar 14, 2017 11:20:00 AM

In the course of patient care, formalin-fixation and paraffin-embedding (FFPE) of biopsy tissue samples are routinely performed, where these samples can be analyzed by histology and archived to link the sample with clinical long-term follow-up. With the development of advanced NGS-based oncology gene panels, it is becoming increasingly important to consider pre-analytic variables when extracting nucleic acids from FFPE-treated samples. This post covers frequently asked questions (FAQs) around the extraction of nucleic acids from FFPE samples for downstream NGS analysis.


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Topics: NGS, next gen sequencing, FFPE Tissue Kit, biopsy tissues, Deparaffinization Solution, Formalin-fixed, paraffin-embedded, nucleic acid, ethanol, molten paraffin

 
 

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