Diagnostic Precision

A SeraCare blog focused on precision medicine and advanced clinical diagnostics

Choose your Article Focus | NGS | Molecular & Serology

The Trouble with Troubleshooting Clinical NGS

Catagory: QC Challenges, QC Management Software, iQ NGS

Posted by Peter Duncan on Nov 15, 2018 11:00:00 AM
Have you ever seen those late night infomercials? One of the things I love whenever I have watched these is the over-the-top acting to depict whatever frustration the target audience must be feeling if they can’t coil up their garden hose or manage their closet space. As frustrated as these actors are, I can’t help but imagine what an actor’s depiction of the level of frustration that a clinical genomics lab director might be feeling when things don’t go according to plan while carrying out an NGS assay. Figure 1 Frustrated Actor in PocketHose Infomercial
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Keep Calm and Standardize On

Catagory: NGS, QC Challenges, QC Management Software, qc management

Posted by Peter Duncan on Oct 5, 2018 10:00:00 AM
There is that old adage that says the only thing that is constant is change. This is one of those universal truths we have all come to accept. Heck, even Dunkin' Donuts, widely credited as being the inventor of the word “Donut,” is dropping the word from their brand name. Blasphemy! But that is for another blog...
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Next-Generation QC Precision Metrics for Next-Generation Sequencing

Catagory: NGS, QC Challenges, NGS Management Software, QC Management Software, bioinformatics, Quality Management Systems, qc management

Posted by Lorn Davis on Apr 16, 2018 4:00:00 PM
Introduction There is an even greater onus to track quality control metrics for NGS assays because of the number of steps and elements that must successfully work together to produce consistent results. The Standards and Guidelines for Validating NGS Bioinformatics, published in January by AMP and CAP, highlight the importance of tracking QC metrics over time because “trends in these metrics can indicate an emerging issue with an NGS process that has not yet manifested itself in failed tests.”
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What Is The Difference Between Quality Control Software Like iQ NGS And LIMS?

Catagory: NGS, QC Challenges, QC Management Software, qc management

Posted by Lorn Davis on Apr 6, 2018 8:00:00 AM
SeraCare Life Sciences, in partnership with GenomeWeb, recently offered this exciting on-demand webinar: 
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Is Your NGS-Based Assay on the Right TRK?

Catagory: NGS, QC Management Software, qc management, reference materials, RNA fusion

Posted by Trevor Brown on Oct 9, 2017 2:13:00 PM
Despite the absence of clear guidelines or firmly established best practices, next-generation sequencing (NGS) assays are becoming the method of choice for gene fusion detection. This is significant because, although some of the cancers that contain fusion RNAs are rare, they’re now treatable thanks to new targeted therapies. If your assay can detect fusion RNAs, it can help profile tumors for important diagnostic, prognostic, and therapeutic targets, which can lead to improved patient outcomes. The old FISH method limited you to one type of fusion variant at a time; it was effective, but also slow and cumbersome. With the latest NGS techniques, detecting fusion RNAs is more efficient than ever. It’s more sensitive and can detect multiple fusions in the same assay. Nevertheless, it’s still challenging because of the complex workflows and the need to rigorously ensure performance across all fusion variants. From extraction, to library prep, to sequencing, to the bioinformatics pipeline, there are countless points where something could go wrong.
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