Diagnostic Precision

A SeraCare blog focused on precision medicine and advanced clinical diagnostics

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Matthew Butler

Recent Posts

If I Call Out of Tune, Would Mutations Stand Up and Walk Out on Me?

Category: SeraSeq, NGS

Posted by Matthew Butler on Aug 9, 2018 12:00:00 AM
One of several important steps in next-generation sequencing (NGS) is tuning the many options provided by mutation callers. Providing values for options configures the signal to noise ratio of the impending mutation calls. In theory, providing values that increase the stringency of mutation calls will reduce the number of false positive calls and thus enrich for true positives. In practice, increasing stringency can eliminate true positives.
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