Diagnostic Precision

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What specifically contributes to high levels of NGS accuracy?

Posted by Dale Yuzuki on Jul 7, 2017

Accuracy of NGS results is extremely critical for clinical genomic laboratories, when developing, validating or running an assay.  At the 2017 Precision Medicine World Conference a panel discussion entitled ‘Achieving Accurate NGS Test Results’ featured Dr. Greg Tsongalis, Director of Clinical Genomics and Advanced Technology at Dartmouth Hitchcock Medical Center;  Dr. Dara Aisner, Associate Professor of Pathology at University of Colorado Anschutz Medical Campus School of Medicine; and Dr. Russell Garlick,

Chief Scientific Officer of SeraCare Life Sciences.

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The individual presentations and ensuing discussion have been edited for clarity and summarized in a document called “Achieving High Levels of Accuracy from Next-Generation Sequencing Assays in Clinical Laboratories." This document lays out:

  • The procedural steps that may introduce variability
  • Key endpoints to focus on to mitigate that variability
  • The importance of proper validation and execution of data analysis and data curation
  • The benefits of inter-laboratory and cross-platform validation

Other topics of interest from this conference have been presented here and Dr. Tsongalis covers the sources of variability in FFPE material in this video interview

Access the 2017 Precision Medicine World Conference document, “Achieving High Levels of Accuracy from Next-Generation Sequencing Assays in Clinical Laboratories” by clicking here

PMWC White Paper Download

Topics: clinical genomics